![]() Beast of Winter DLC - heed the call of Vatnir, a priest of the god of death, to halt the tide of destruction encroaching upon the living.Witness their personal relationships, reactions to player choice, and interactions with each other unfold. Assign multiple classes and deeper abilities for each. Gather your party: Build your party and customize your companions to join you on your quest.Play however you want: Detailed character creator character and over 1000 class permutations gives you the freedom to be whoever you want.A massive adventure: Over 100 hours of gameplay per playthrough. ![]() Play in either Turn-Based Mode or Classic Real-Time with Pause: Immerse yourself in a deeper single player RPG game experience - now with both classic real-time with pause and new turn-based combat, Deadfire builds on the foundation of classic tabletop gameplay more than ever before. ![]() Now resurrected, it is up to you and your companions to use magic, gunpowder, and steel to uncover the rogue god's machinations as he tramples across the Deadfire Archipelago.īend the world to your will as you explore the depths of infinite possibilities, including detailed character customization, total freedom of exploration, and more meaningful choices at every turn.įeaturing an enormous open world, a massive cast of characters, and a story you can play your way, Pillars of Eternity II: Deadfire Ultimate Edition redefines the role-playing game experience for a new generation. The god Eothas awakens from his slumber, killing you and stealing a piece of your soul. ![]() The award-winning RPG from the masters at Obsidian Entertainment finally arrives on Xbox One, featuring all major updates and expansions. ![]()
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![]() ![]() Here, the interviewer is likely looking for information about the new keyword. Each object has a state in which all the member variables have specific values. While a class is a logical entity, an object is a physical entity. This is a variation of the previous question that forces the interviewee to define both classes and objects.Ī class is a template used for the creation of objects whereas an object is an instance of a class. You can learn more about classes and objects in our tutorial: Classes and Objects in Java What is the difference between a class and an object? A class can contain fields and methods to describe the state and behavior of an object. A class is a blueprint from which individual objects are created. They are so fundamental that you really can’t write even the most basic program without one. In an OOP language like Java, classes are the bedrock. You can learn more about popular Java IDEs in our roundup: The Top Java IDEs. NetBeans is another good choice.īecoming familiar with Java developer tools like application performance monitors and collaboration tools is also a good idea. If not, take some time to get acquainted with the top two – Eclipse and IntelliJ IDEA – at the very least. Hopefully you have used at least one IDE prior to your interview. You can learn more about OOP concepts in the following tutorial: What is Object-oriented programming in Java? Which Java IDE Do You Use?Īn IDE (or Integrated Development Environment) is a software application that increases developer productivity by combining capabilities such as software editing, building, testing, and packaging in an easy-to-use application. The user can create additional threads by extending the thread class or by implementing the Runnable interface. JVM creates a thread which is called the main thread.
![]() ![]() After 30 days, we again delete the “Elicenser control center”, “Cubase Elements 8”, and demolish this file “C: \ ProgramData \ Syncrosoft \ l”, and restart the computer. – This homunculus will work only for 30 days! – How to remove all licenses in “Soft-Elicenser” and reinstall the trial version of “Cubase Elements 8”: 2. Install (CubaseElements8TRIAL.zip) the trial version of “Cubase Elements 8” from under the “Start Center”. Install the “Elicenser control center” – if it is not yet available of course. Disable steinberg hub in preferences / general - Install the trial version of Cubase Elements 8: 0. ![]() Start Cubase 8 and select Cubase Artist 8. Disconnect possible Steinberg USB-Elicenser if it contains Cubase trials / licenses. Replace and copy files to C: \ Program Files \ Steinberg \ Cubase 8 files in Cubase Elements 8. Update Cubase to 8.0.40 with updater from Cubase 8.0 site 3. Install Cubase 8.0 64-bit from the Start Center in Cubase 8.0 for example from Steinberg Cubase 8.0 site or elsewhere. repeat 2 and 3 after 30 days – there are ways to get unlimited trial license Install Artist: 1. ![]() Install again ELement 8 trial from Start Center only after restart computer 4. After 30 days uninstall Elicense control center, uninstall Elements 8, delete C: \ ProgramData \ Syncrosoft \ l, restart computer 3. Install (CubaseElements8TRIAL.zip) Cubase Elements 8 trial from Start Center only – Works 30 days this way – To erase all licenses in Soft-Elicenser and reinstall Cubase Elements 8 trial: 2. Install Elicenser control center if it has not been installed already from elicenser site 1. ![]() ![]() ![]() The Supreme Court upheld the trial judgement in favour of the plaintiff awarding disgorgement with respect to Strother’s personal financial gains earned as a result of his conflict of interest, even though the plaintiff had suffered no provable loss. The plaintiff had sued their former lawyer, Strother, for breach of confidence and breach of fiduciary duty. Strotheris another leading Supreme Court decision on disgorgement, looking this time at the remedy in the context of fiduciary relationships. Babstock held that “courts should consider whether the plaintiff had a legitimate interest in preventing the defendant’s profit-making activity” and that disgorgement is appropriate where the claimant’s interest “cannot be vindicated by other forms of relief”.Ītlantic Lottery is the Supreme Court’s most recent pronouncement on the issue of disgorgement. The Supreme Court in Atlantic Lottery Corp Inc. The remedy has also been awarded for breach of contract in exceptional circumstances, where damages are inadequate and where the circumstances of the case warrant such an award. Disgorgement is also ordered in cases involving corporate oppression where it would have been unjust that the responsible party benefit from the oppressive conduct. Disgorgement is most commonly awarded as a remedy for breach of fiduciary duty and breach of trust. When is disgorgement available?ĭisgorgement requires a wrongdoer to give up the wrongfully-acquired gains. This remedy may be an alternative to compensatory damages in fraud cases. Where a wrongdoer’s profits are so intimately connected with the wrong and these profits would not have been earned but for the wrongful acts, a plaintiff may turn to gain-based disgorgement remedy as a more appropriate measure of damages.ĭisgorgement aims to deprive a wrongdoer of his or her ill-gotten gains and has the practical effect of transferring the wrongfully-obtained profits. For example, a wrongdoer who improperly uses trust funds, profits from that breach of trust, and later returns the monies to the trust account, but seeks to keep the gains. In some cases, a plaintiff may have suffered no damages, but the defendant has gained significantly. However, this does not always fit the circumstances of the breach. When a plaintiff suffers a loss due to the misconduct of a defendant, the typical approach is to award damages that reflects the loss. ![]() ![]() ![]() What can I do with the Free PDF Editor app?Īs mentioned earlier, you can use the Free PDF Editor to create, edit, and save PDFs. You can also fill color into whichever shape you choose. After you insert the shape, you can edit the border’s size, type, and color. These shapes could be anything, from a rectangle to a square or an ellipse to a triangle. In addition to this, the PDF editor also lets you add different shapes to your documents. You can easily resize any image or crop it to suit your preference. The application supports all popular image formats, including JPEG, PNG, BMP, GIF, and ICO. Similarly, the Free PDF Editor app has an insert image option that lets you upload and paste pictures onto a PDF file. You can then type or paste the content you want and change its font, size, style, and color. ![]() Once you do that, you’ll see an input box that you can drop anywhere on the page and later drag into position. Nevertheless, if you download and use this tool to create PDFs, you can easily edit those without facing any trouble.įor starters, if you wish to add text to a PDF file, all you need to do is click on the ‘Insert Text’ option available on the primary menu of the Free PDF Editor app. You can’t edit imported documents with this program. While the name clearly says Free PDF Editor, it is an editor for only PDFs created by the application. If you’re looking to edit PDF files with this program, you will be disappointed. The Free PDF Editor often only opens the PDFs that it creates all other files may lead to the application crashing. However, if your try opening another PDF created from another application, you will face some difficulty. After you’ve completed the document, simply click on save and share the PDF with others. Once that’s done, all you have to do is drag them around and resize them according to your preference. You can use your mouse to drop these elements onto the surface of the page. These elements include options like text, shapes, images, and more. You can start by creating a document and then insert different elements. ![]() ![]() However, when it comes to its interface, the design looks like an old Windows application. Once you complete the download for Free PDF Editor, you get access to a simple application that is easy to use. As an added benefit, you can easily take the application along with you on a USB disk. Instead, you can directly run the executable file and get started with the application. This means that once you download the application, you don’t need to install it on your Windows computer. One of the best parts about downloading Free PDF Editor is that it is portable. So far I can’t find any solution within Acrobat Reader.Is the Free PDF Editor app easy to install? It would make a massive difference to the speed of mark-up across a document if it could be done. It currently needs to be frequently ‘re-set’ to use a different color. It would save a lot of time and there would be a lot less playing about with keyboard shortcuts and mouse clicks to carry out the task. If that is too much for the ‘system’, then at least 3 different ones would still be helpful. Ideally I’d like to have 5 ready-to-go ‘highlight text’ icons at the top of the document (with different colors for each that I have pre-chosen). When using Acrobat Reader to research articles I frequently need to highlight a variety of sentences (within one document) in different colors, for example one color for statements I agree with, a different color for statements I disagree with, another for statements that prompt me to ask a question, etc. ![]() ![]() Our time with Eatcake was enjoyable but more importantly they spent time working us out and asking the right questions.įor us the results – website and associated design work, is perfect. Key to us was finally getting an online identity that matched what we were really about – somehow making it entirely authentic and getting away from a website that is what we think people should see. We had 2 beauty parades, with a range of options, from a website from £90,000+ through to £2,500 (and although highly recommended they did not want to meet!) Given the huge number of potential suppliers, this might be an easy process? Not in our case. " Like lots of these things, we rue the time it took to get a recommendation to speak to Eatcake! We have a fast growing company and also recognised that it was a fast changing world so we wanted someone good to help us design a new website and help with a brand/image refresh. We used the identity to inform the interior design of their refitted office space. They asked Eatcake Design to update their corporate identity and refresh and reorganise their website. Louise Minchin, Brandon Trust, Shops Locality ManagerĮdward Drummond are a recruitment and headhunting agency that operates globally. I also felt that Jon had a connection to what we were doing as an organisation which also made a difference to the way we worked.Įatcake have also helped us to create leaflets, branding and wall art for our shops, all of which have been exactly what we've asked for and I would absolutely recommend them to other organisations – thanks guys!" ![]() He was also a great support to me, working on a project of this scale for the first time I needed all the guidance I could get, and I really felt listened to when we had to compromise design and practicality. Jon respected my expertise and I felt confident in his and we came to some great solutions. I loved working with Jon because his vision of what the shop could be was so similar to my own, we even had similar mood boards at our first meeting, and I knew we would create something exciting together! We get complimented on the quality of our stock and the way in which it is presented in the surroundings we have created on a daily basis, and that all started with this shop!įrom our exposed fixtures and stripped back feature wall, industrial fittings and retro lighting, and our signature 'Big B' light box, the shop is full of themes and touches that we have kept running through our subsequent shops and continue to make us stand out from the crowd. ![]() Together I think we have shown the good people of Bristol that donated goods can be displayed in an interesting and modern way, and there's nothing to stop us being just as on trend and innovative as other high street retailers. They helped us to set the tone and style of our pilot store, with contemporary twists and ideas that challenged what people expected of us as a charity. "Eatcake were involved with the design of our first shop which opened on Cotham Hill in 2015. Brandon Trust wanted to enter this competitive market and asked Eatcake Design to help them apply their branding effectively.Ĭollaborating with a superb and creative retail team, we raised the bar for the shopping experience in the charity sector. The charity shops of Gloucester Road and Cotham Hill in Bristol are some of the best and busiest in the country. Viki Browne, Artistic Director, Many MindsĮatcake Design worked with longstanding client Brandon Trust to develop a distinctive theme for their new shops. ![]() They were able to turn our energy and excitement into a logo that felt like it really communicated what we are about." One in particular stood out for us, It was perfect. They came back to us with multiple options, all of which felt really in keeping with our brand. As a new charity this could have been difficult to articulate but we were made to feel welcome and relaxed as we were invited to share with them what excited us about the work we do. They listened to what we wanted and asked questions so that they were able to get a feel of what we did, what we needed from the logo and who it was for. A safe structure in which an element of chaos and anarchy can be expressed. The logo demonstrates that the charity operates in a grassroots, non-hierarchical and collaborative way and the work is often full of play and experimentation. Many Minds use performance as a way for people experiencing mental ill health to improve confidence, self esteem and social networks in a creative environment. New Bristol charity Many Minds asked us to design them a logo. ![]() ![]() ![]() He rose to stardom with the release of the song “Iron Man”. Kizz Daniel “Buga” Lyrics Meaning Profileĭivine Ikubor (born ), popularly known by his stage name Rema is a Nigerian rapper, singer, songwritter and stage perfomer. Here is “Igbo and Shayo” Meaning by Burna Boy Did she agree or decline Rema’s love interest? Did Rema get over his supposed lockdown? Our questions on the song are unanswered.īut then that’s the whole point of the song. However, at the end of “Calm down“, we are not told if she agreed or not. Rema tells us literally everything about the wooing process, he even gets detailed to you the point of telling us her dress color- Yellow. The only thing that seems like a flaw with Rema’s “Calm down” is incomplete storytelling. I mean, apart from the fact that Selena Gomez’s voice is so angelic, her verse on Calm Down is so thrilling that we want to hear more of her. But the storytelling makes it an intriguing one.īoth Rema and Selena Gomez delivered a bop! “Calm down” would have been a boring listen. If Rema did something else other than this on “Calm down“. This storytelling plot on Calm down lyrics meaning makes the song hold you down till it ends. He says “ other girls commot like chewing gum” but the girl is permanent. He even discusses how he met her in a party and apart from her body, he says her character of being “ Mellow” rather than other girls doing “ too much” is what attracts him. “If you leave me, I no go fall in love again”Ĭalm Down Lyrics Meaning shows how Rema employs storytelling to get us interested in the song. ![]() Meanwhile, in Rema’s defense, the lady should “ Calm down” and trust him because there are actually more girls who want him but he doesn’t like them like he does the girl. So yes, Rema’s “Calm down” is very relatable. They can never “Calm down” while showing the lady that they desire her so much. It is the point of attraction for most guys, and in “Calm down“, Rema asserts it is what has put him on “Lockdown“.īeing on “ Lockdown” is the default for most guys when they want a lady. He says the lady’s body puts him on “ Lockdown” because he can’t do anything else but think of her- or her body in this case.Ĭalm down lyrics meaning shows it is a relatable song because it happens that what most guys observe first before even seeing a lady’s character is her body. Which means if he sees another lady with better “qualities“, he would “fall” in love with her too. Therefore, it may be right for her to not express so much trust in Rema since her body is his attraction. He even says he can’t take a “no” from her. He uses descriptive lyrics to explain how desirable her body is and how he wants it no matter what because he can’t get his mind off her. On “ Calm down”, Rema expresses that he loves the girl in question and that he wants her. “ If I tell you say I love you, no dey do iyanga” On “Calm down“, Rema tries to allay her fears by telling her that she should “Calm down” and not feel all “Insecure” because: Therefore, Rema implores her to “Calm Down” and listen to him. However, the issue here is trust- Which the lady believes Rema does not possess. “ Calm down” is a song about Rema- or the singer’s persona in this case, seeking a lady’s love interest. Here is Calm Down Lyrics Meaning Rema ft Selena Gomez Share to Friends Rema Calm Down Lyrics Meaning ![]() ![]() ![]() As the player, you won’t have the option to peep inside chests, closets, or some other sort of capacity units inside a resident’s home. There is no fixed time for the locals to rest and the house likewise remains bolted while they are dozing. You can go inside the place of a resident on the off chance that they are inside. The Animal Crossing New Leaf Amiibo update can contact wisp which permits them to move in any resident utilizing their amiibo card. You can’t know whether they have persuaded a resident from another town to move in, or the player has persuaded the resident to move in from the campground. It is highly unlikely to figure out which resident will move in next except if you see a house set up. If I somehow happened to outline the various classifications of characters for you it would resemble:ĪCNL or Animal Crossing Character’s Function:Įssentially, how animal crossing residents work is that they move into the town at arbitrary. Other than being inhabitants, characters can likewise be retailers. The animal crossing characters are essentially various types of animals that can move into your town which would imply that they are inhabitants. Animal Crossing Characters :Īnother interesting element of animal crossing is the animal crossing locals which are essentially the characters of the game. In ES 2011, data was uncovered to individuals and mechanics of the game were expounded extensively. At first, the game Animal Crossing New Leaf was first declared at E3 2010. Being the most recent passage in an establishment that began the N64 (Japan-just) in 2001, animal crossing new lead has increased across the board prevalence in the gaming scene. Realizing how to dominate the match, I am going to cover probably the best tips for ACNL game including some astounding animal crossing new leaf swindles.Īnimal Crossing New Leaf is a real existence recreation based game exceptionally intended for the handheld Nintendo 3DS support. It resembles the ideal amusement for you and winds up lighting up your day. Even though the idea of animals and bugs may appear to be anything but difficult to you from the start, there is an enormous number of grown-ups who have played the game and have had issues with numerous parts of the game. Truth be told, you will feel frustrated at whatever point you should quit playing the game and continue ahead with your genuine, dull life. I can guarantee you that you won’t be even the slightest bit frustrated to play this game. The game gives you a similar involvement with which you get the chance to meet various types of animals, get the bugs that you don’t care for and swing the net over some unfortunate fish too.īefore we can cover the significant focuses in animal crossing new leaf guide like ACNL face guide, ACNL hair guide, animal crossing townspeople, ACNL fish guide, animal crossing bug guide, ACNL Halloween, ACNL Coffee Guide, animal crossing new leaf face guide, animal crossing new leaf hair guide, ACNL room thoughts, animal crossing new leaf cheats, ACNL craftsmanship guide and so on, let me give you the nuts and bolts. Because of the topical landscape of animals and their natural surroundings, this game is brimming with fascinating characters like animals and bugs who wake up on your handheld Nintendo. Animal Crossing New Leaf is an adaptation of the game arrangement of the great establishment of Animal Crossing possessed by Nintendo for the handheld gadgets claimed by numerous excited individuals everywhere throughout the world. Animal Crossing New Leaf is unquestionably not one of them and has figured out how to win the hearts of a large number of players around the world. ![]() Very few games these days can prevail upon the hearts of individuals all over the world. ![]() You’ll be amazed to realize that this game is perhaps the best one on the entirety of your Nintendo supports. Additionally, I will likewise examine the Animal Crossing locals as it is imperative to know the well known ACNL townspeople and different characters. That is the reason I have chosen to abridge and gather the best tips expected to win right now New Leaf Complete guide including ACNL Hair Guide, ACNL Face Guide, ACNL Coffee Guide and the sky is the limit from there. It wouldn’t bode well for you to play Animal Crossing New Leaf without understanding the game, particularly since understanding this game all alone isn’t a simple errand. ![]() ![]() ![]() The earliest documented use of cells grown for scientific purposes dates back to the late nineteenth century. So, why would we want to grow immobilized rather than free cells, or vice versa? As you might imagine, there are advantages and disadvantages to culturing cells in a free or immobilized state depending on the application - and therein lies the real difference. Free cells are just that - free and able to move around while growing. At the most basic level, immobilized cells differ from free cells in that they grow while adhered to a substrate. Hello Yvonne, It sounds like you are interested in learning more about the different ways in which cells and microorganisms can be grown. You may be able to devise a cloning strategy that requires the use of every one of these DNA modifying enzymes, but you should also ask yourself why? When devising a cloning strategy, the simplest approach is oftentimes the best.įor more information on molecular cloning, please check out the following links: With cloning, as with many things in life, there exists an infinite number of ways to go about doing things. If the DNA insert lacks 5’-phosphates, it would need to be treated with PNK so as to be ligated with a CIP-treated vector. Blunt cloning strategies typically involve the use of T4 DNA ligase, and the cloning vector is often treated with CIP in order to prevent vector re-ligation. Instead, Klenow would be the enzyme of choice for generating blunt ends from DNA templates with overhangs. As we have already noted, TDT is not often used in cloning experiments and it is more likely to generate 3’ overhangs than blunt ends. Now, on to your question: could a blunt cloning strategy be devised that uses all these enzymes? Most likely, no. ![]() “CIP-ing” a vector after it is digested, but before it is used in a ligation reaction with another DNA fragment, is a very common technique used to reduce the formation of re-ligated vectors that lack an insert. Contrastingly, CIP is often used in cloning to prevent the re-ligation of a cloning vector that has complementary DNA ends - in this way, the vector will not have any free 5’-phosphates and won’t be able to re-ligate with itself. Therefore, PNK is often used to attach a 5’-phosphate to an insert that lacks a 5’-phosphate. T4 DNA ligase requires a 5’-phosphate on at least one of the two DNA fragments it joins. In contrast, calf alkaline phosphatase (CIP) removes the 5’-phosphate groups from DNA fragments. T4 polynucleotide kinase (PNK), also derived from bacteriophage T4, is used to transfer the gamma phosphate from ATP to the 5’-OH of a DNA fragment. How does T4 DNA ligase work? It forms a covalent phosphodiester bond between a 5’-phosphate group on one DNA molecule and a 3’-hydroxyl group on another DNA molecule, using ATP to carry out this task. T4 DNA ligase acts like a molecular glue that allows molecular biologists to easily insert a gene of interest into a plasmid vector. If CoCl2 is added to the reaction, TDT can often label any type of 3’ end (3’ overhangs, 5’ overhangs, and even blunt ends!).Īs its name suggests, T4 DNA ligase (derived from bacteriophage T4) ligates DNA together. While it can be used for cloning purposes, it is most commonly used for end-labeling (i.e., to attach labeled nucleotides to the 3’ end of a DNA molecule of interest). Terminal transferase, also known as TDT, is a type of DNA polymerase that adds nucleotides to the free 3’ ends of single-strand DNA. For cloning purposes, Klenow is often used to extend recessed 3’ ends of DNA fragments, something you may need to do if you wanted to ligate two DNA fragments with blunt ends (called blunt cloning). Klenow exhibits polymerase activity but lacks exonuclease activity. Klenow is a protein fragment derived from E. We’ll then address your question about how these enzymes could be used in blunt cloning experiments. Hi Girisaran, Let’s start by taking a look at your list of DNA modifying enzymes and discussing how each might be used in molecular cloning experiments. ![]() ![]() ![]() ![]() Weekdays any orders placed up to midnight will be available for collection the following day. Our store is open Monday to Friday 8.00am - 5.00pm and Saturday 8.00am - 12.00pm so any orders placed the remainder of the weekend will be available for collection on Monday. If your item is in stock you will still be able to do a same day collection and payment. After the cut off you are welcome to do a Call and Collect order by phoning our trade counter on 01502 507666. After that the product will be ready the next day for collection. Our cut off for same day collections is 3.00pm Mon-Fri / 11.00am Saturday. If your item is showing "In Stock" then you will be able to do a Same Day Click and Collect order from our Lowestoft Pinbush Road. Is My Item Available for Click and Collect at your Lowestoft Store? The appropriate tracking can be provided when necessary.ĭetails of each courier service we offer are detailed in our Delivery Guide. If you are an international customer, your goods will initially be sent via UK Mail/DHL, and then exported to either Eurodis or DHL. If your item is being delivered in the UK or Ireland, it will be delivered by UK Mail/DHL, Hermes or Royal Mail, with the exception of pallet items. What courier will my item be delivered with? You will have the option to choose this service if you are purchasing your item for delivery to mainland UK. This will be highlighted by a green tick and a 1 day dispatch time on the item information. If your item is stocked in our immediate picking location it will available for you to choose a next day delivery. Is My Item Available for Next Day Delivery? In addition we now deliver through Europe on a 48 Hour Delivery Service as Standardīelgium, Germany, Luxembourg, France, Netherlands, Rep Ireland.Īustria, Denmark, Czech Republic, Poland, Lithuania, Hungary.Įstonia, Spain, Sweden, Italy, Portugal, Latvia. £36.00 - Pallet Delivery (2-3 Days Approx - selected products only)Īlternatively if you view your parcel once items have been added and enter your postcode you will see what derlivery services are available. £8.99 - Saturday Delivery (Orders placed before 3.00pm Friday) *orders placed after 3.00pm Friday will still be delivered Monday if ordered before 11am Saturday Morning £2.99 - Next Day UK Delivery (Orders placed before 3.00pm Monday - Friday) £0.00 - Standard UK Delivery (2-3 Days Approx) In additional to our standard service we also offer some premium delivery services if you need your item quickly For full details visit our Delivery Guide. Surcharges can apply to certain postcodes depending on the service type. Standard delivery is free with no minimum order charge to most mainland UK delivery addresses. In addition we can now also deliver through Europe. ![]() Buyaparcel offer delivery throughout the United Kingdom, including Northern Ireland, Scottish Highlands and other Islands of the UK. ![]() |
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